Watch our presentation at the Biotech Week Boston 2023 conference on “An Improved Method of Characterization of Ultrafiltration (UF) Membranes for Bioprocessing”, given by Thomas D. Lazzara, Ph.D., Director of Research and Development.
The characterization of ultrafiltration membranes with molecular weight (Mw) markers is a standard method to determine the retention rating of membranes in terms of kDa, (i.e. the molecular weight cut-off [MWCO]). Typically, a single marker is used, or alternatively a combination of two distinctly different Mw markers with varying sizes are used to assign a pore size rating. With SepraPor® UF hollow fiber filter membranes, we developed an improved method of determining the MWCO using a series of polymers of increasing Mw. This continuum approach provides for well-characterized ultrafiltration membranes with improved lot-to-lot consistency. Each MWCO membrane manufactured and characterized using the new method features a specific retention range. This higher standard level of membrane characterization has already driven the implementation of unique process control strategies to the hollow fiber manufacturing process, resulting in enhanced lot-to-lot reproducibility. Well-characterized membranes with improved consistency can improve the performance of a purification process. To highlight the performance of SepraPor® ultrafiltration membranes, a UF/DF case study comparing UF hollow fibers to cassettes and demonstrating the optimization that can be achieved during plasmid DNA purification will be presented.
Dr. Lazzara discusses the benefits Meissner’s unique method of characterization in an interview with Genetic Engineering News (GEN).
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